Use of the mgb eclipse system and smartcycler pcr for. Who prequalification of in vitro diagnostics public report. Cepheid warrants that the smartcycler processing block the instrument i is free from. By automating the entire amplification and detection process, the smartcycler system can deliver highly accurate and consistent test results from prepared biological samples in 3040 minutes. The optical system of the unit is centered around the icore, a solid state module which does not require warm up. Comparison of smartcycler realtime reverse transcriptionpcr. Cepheid officially launched the fullyautomated genexpert system into the clinical market with the release of xpert gbs, a test for intrapartum screening of group b streptococcus in labor and delivery, in 2005.
Mics user friendly software is packed full of intelligent analysis features including. The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the. Commercial off the shelf cots smartcycler gene expression analysis researchers can receive results within 24 hours of experiment run onorbit data can be used to guide the experiment in.
The test can provide a highly accurate result in as little as 30 minutes, during the critical period. Evaluation of cepheid smartcycler and euroclone duplicareal time dual easy ct assay for confirmation of chlamydia trachomatis. Cepheid is a wooden kinetic sculpture named after pulsating stars. Pdf phoma tracheiphila is the causal agent of a tracheomycotic disease of citrus. Shorter annealing times favor specificity and minimize formation of. The smart cycler ii system employs realtime pcr for anthrax detection.
Main focus of gene quantification web page is to describe and summarize all technical aspects involved in quantitative gene expression analysis using realtime rtpcr and competitive rtpcr. Find and purchase cepheid smartcycler products at invitrogen life science technologies. Using quantification cycle values extracted from qpcr instruments. Its curvy, raylike forms slice and meld into each other as they spin and rock in a seemingly random motion.
The cepheid smartcycler ii is a modular real time pcr system. Pdf evaluation of the portable cepheid smartcycler real. Since then, 20 fdacleared tests and 27 ceivd tests have been introduced from healthcare associated infections to womens health, sexual health, and virology. Click the resources tab to download rest 2009 software free of charge. Smartcycler ii pcr thermocycler cepheid, sunnyvale, ca capable of performing cycling parameters described below and simultaneous realtime sequence detection for fam, texas red, and cy5 dyes. Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. The smartcycler system is highly robust and compact, with no moving componentsideally suited for mobile use in fieldtesting applications. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the. Evaluation of the cepheid smartcycler realtime pcr machine for rapid and portable diagnosis of footandmouth disease find, read. Laboratory considerations for use of cepheid xpert mtbrif. The equipment was switched on and appropriate thermal profile was selected prior to preparation of the reaction mix. Using quantification cycle values extracted from qpcr instruments, it uses a proven and universally applicable model to give finalized quantification resu. Qiagens new realtime pcr cycler, the rotorgene q, provides outstanding. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing.
Available realtime rtpcr cycler and kinetic pcr machines. For detection and discrimination of influenza a virus, influenza b virus and respiratory syncytial virus. This is a 510k application for a new qualitative realtime polymerase chain reaction pcr assay used with the cepheid. Quick reference for realtime rtpcr influenza a assays for subtype h5 or h7 detection for ab7500 fast and cepheid. What assay is optimal for the diagnosis of measles virus infection. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. As testing volumes expand, you can add more capacity to the system at any time. The assay was performed with the cepheid sunnyvale, calif. The its smartcycler pcr assay described is designed for use in the clinical microbiology laboratory as a component of the culture algorithm for the identification of mycobacterium species. Cepheid enabling access to molecular diagnostic testing.
Evaluation of the smartcycler ii system for realtime. Cepheid smartcycler ii thermal cycler automated realtime. You can configure the smartcycler system to suit your exact needs. Realtime pcr is the continuous collection of fluorescent signal from one or more polymerase chain reactions over a range of cycles. Rapid and sensitive detection of mycobacterium dna using. Cepheid s smart cycler instrument facilitates the speed and accuracy of the idistrep b test. Evaluation of the portable cepheid smartcycler realtime. Evaluation of the cepheid smartcycler realtime pcr. This study evaluated the performance characteristics of the cepheid. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical. Download user guide rest 2009 software user guide english pdf.
Cepheid is an american molecular diagnostics company that develops and manufactures fully integrated medical testing systems. Pdf identification and detection of phoma tracheiphila, causal. Click on the smart cycler icon to start the life science 2d smartcycler software. C, june 2012 the end user license agreement is located at c. Protocol confirmation of salmonella species isolates by. The smartcycler ii system demonstrated its wide potential for detecting adenovirus, hsv1, vzv, and c trachomatis in a small laboratory setting in comparison with a large central molecular. Smartcycler instrument in a protocol comprising rt and pcr. Cepheid smartcycler system software license agreement license grant.
Smartcycler system is a leading realtime pcr testing platform for research hospitals, university labs and government agencies. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. You are also free to make these changing later on while analysing your run. An evaluation of the performance of a measles virus realtime reverse transcriptase pcr using the cepheid.
By automating the entire amplification and detection process, the. H7 and salmonella species in spent sprout irrigation water or sprouts october 2015, version 1. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid. Optimizing and analyzing realtime assays on the smartcycler ii. The smart cycler ii system employs realtime pcr for anthrax. Headquartered in sunnyvale, california, the companys systems enable rapid. This is a new 510k application for a qualitative realtime reverse transcriptionpolymerase chain reaction rtpcr assay used with the cepheid. Rapid and sensitive detection of mycobacterium dna using cepheid smartcycler. Manualslib has more than 2 genexpert manuals checkout popular genexpert categories medical equipment manuals.
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